Journal: Journal of Advanced Research

Article Title: Novel function of macrophage migration inhibitory factor in regulating post-infarct inflammation and the therapeutic significance

doi: 10.1016/j.jare.2025.05.030

Figure Lengend Snippet: Fibroblast function in chimeric mice with specific somatic cell-derived MIF ( WT KO ) and bone marrow cell-derived MIF ( KO WT ) after myocardial infarction (MI). A-D, Changes in mRNA level of collagen 1 (Col-1), Col-3, α-smooth muscle actin (α-SMA) and transforming growth factor-β (TGF-β) in the infarct tissue of WT KO and KO WT mice at 6 days post-MI. n = 4–5 per group. E, Representative images of α-SMA positive stained cells in the infarct region. The cyan color (white arrow) indicates overlap of α-SMA staining (green) with DAPI (blue) staining for nuclei. The amplified image (inset) shows a typical overlap staining. F, Quantification of α-SMA positive cells in the infarct region from WT KO and KO WT mice at 6 days post-MI. n = 3–4 per group. G, Proliferation of primary mouse fibroblasts isolated from sham, 2-day MI and 6-day MI hearts. n = 5–6 per group * P < 0.05 vs. sham in the same genotype. ** P < 0.01 vs. sham in the same genotype. *** P < 0.001 vs. sham in the same genotype. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: LV sections were incubated with rabbit anti-mouse α-SMA (1:200, Cell Signaling Technology, USA), followed by secondary antibody Alexa Fluor® 488 goat anti-rabbit IgG (1:200 green florescence, Abcam).

Techniques: Derivative Assay, Staining, Amplification, Isolation

Journal: Stem Cell Reviews and Reports

Article Title: Investigating the Therapeutic Efficacy of Quality-Controlled, miR-146a-5p-Enriched Small Extracellular Vesicles Derived From MSCs Against Idiopathic Pulmonary Fibrosis

doi: 10.1007/s12015-025-10976-8

Figure Lengend Snippet: UC-sEvs alleviate fibrosis-related destruction of lung structure and function. A Representative images of lung sections stained with HE, Masson’s trichrome, and Sirius red. Scale bar = 100 μm. B Lung fibrosis was measured on day 26 on the basis of Ashcroft scores. C Comparison of the lung dry/wet weight ratios among the three groups of mice; n = 6 per group. The data are presented as the means ± SDs. n = 6 per group. Each symbol represents 1 mouse. * P < 0.05, ** P < 0.01, and **** P < 0.0001; D and E Representative immunostaining of lung sections for CD68 (green), CD31 (green), and α-SMA (red); scale bar = 100 μm. Nuclei were stained with DAPI (blue). The red box represents the enlarged area. NC = negative control

Article Snippet: The slides were incubated with primary antibodies against rat anti-mouse CD68 (ab53444, Abcam, UK), rabbit anti-mouse α-smooth muscle actin (α-SMA) (19245, CST, USA), and rat anti-mouse CD31 (ab7388, Abcam, UK).

Techniques: Staining, Comparison, Immunostaining, Negative Control

Journal: Stem Cell Reviews and Reports

Article Title: Investigating the Therapeutic Efficacy of Quality-Controlled, miR-146a-5p-Enriched Small Extracellular Vesicles Derived From MSCs Against Idiopathic Pulmonary Fibrosis

doi: 10.1007/s12015-025-10976-8

Figure Lengend Snippet: The miR-146a-5p/TRAF6/IRAK1 axis inhibited inflammation and the fibrosis response in cell models and lung tissues. A Changes in miR-146a-5p expression in sEvs derived from UCMSCs after a miR-146a-5p mimic or inhibitor was added exogenously. The inflammatory cell model was established by exposing MLE-12 cells to BLM. ELISA was performed for IL-6 B and IL-1β C after treatment with different sEv types. sEv = UC-sEv; sEv-FT = UC-sEv with repeated freeze–thaw cycles; sEv-miR146a + = UC-sEv with upregulated miR-146a-5p expression; sEv-miR146a- = UC-sEv with downregulated miR-146a-5p expression. D Western blot analysis of the protein levels of fibrosis-related factors. α-SMA expression in the TGF-β1-stimulated MLE-12 cells supplemented with different sEv. E Statistical analysis of the expression levels of α-SMA normalized to those of α-tubulin. qPCR analysis of miR-146a-5p F and the target genes traf6 G and irak1 H . The data are presented as the means ± SDs. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001; ns = not significant; NC = negative control

Article Snippet: The slides were incubated with primary antibodies against rat anti-mouse CD68 (ab53444, Abcam, UK), rabbit anti-mouse α-smooth muscle actin (α-SMA) (19245, CST, USA), and rat anti-mouse CD31 (ab7388, Abcam, UK).

Techniques: Expressing, Derivative Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Negative Control

Journal: Cardiology Plus

Article Title: Cepharanthine aggravated atherosclerosis and liver injury in Apoe −/− and Ldlr −/− mice

doi: 10.1097/cp9.0000000000000109

Figure Lengend Snippet: Figure 3. Cep induced liver damage. A, Serum levels of AST and ALT in Apoe−/− and Ldlr−/− mice treated with or without Cep. n = 9 for each group in Apoe−/− mice; n = 8 for the Veh group and n = 6 for the Cep group in Ldlr−/− mice; B, Gross images of liver from Apoe−/− and Ldlr−/− mice treated with or without Cep. n = 6 for the Veh group and n = 7 for the Cep group in Apoe−/− mice; n = 8 for the Veh group and n = 6 for the Cep group in Ldlr−/− mice. Scale bar: 1 cm; C, Liver weight and liver weight index in Apoe−/− and Ldlr−/− mice treated with or without Cep; D, H&E staining of livers from Apoe−/− and Ldlr−/− mice treated with or without Cep. Both internal and peripheral liver sections were captured. n = 9 for the Veh group and n = 8 for the Cep group in Apoe−/− mice; n = 7 for the Veh group and n = 6 for the Cep group in Ldlr−/− mice. Scale bar: 20 μm; E, Sirius red staining of liver sections from Apoe−/− and Ldlr−/− mice treated with or without Cep. Internal and peripheral liver sections were captured. Scale bar: 50 μm; F, Immunofluorescence staining of COL1A1 in liver sections from Apoe−/− and Ldlr−/− mice. Scale bar: 50 μm. n = 5 for each group; G, Immunofluorescence staining of α-SMA in liver sections from Apoe−/− and Ldlr−/− mice liver. Scale bar: 100 μm. n = 5 for each group. All data are presented as the means ± SD. Data for ALT in Apoe−/− mice, and AST and ALT in Ldlr−/− mice were analyzed using the Kolmogorov- Smirnov test. Data for AST in Apoe−/− mice were analyzed using an unpaired Student t test. For C, liver weight in Apoe−/− mice was analyzed using the Kolmogorov-Smirnov test; liver weight index in Apoe−/− mice, and liver weight and liver weight index in Ldlr−/− mice were analyzed using an unpaired Student t test. ALT: alanine aminotransferase; AST: aspartate aminotransferase; Cep: cepharanthine; COL1A1: collagen type I alpha 1; DAPI: 4’-6-diamidino-2- phenylindole; H&E: hematoxylin and eosin; SD: standard deviation; α-SMA: alpha-smooth muscle actin; Veh: vehicle.

Article Snippet: Primary antibodies include rabbit anti-mouse α-SMA antibody (14395-1-AP; Proteintech, Rosemont, Illinois, USA) and collagen type I monoclonal antibody (67288-1- Ig; Proteintech), both diluted at 1:500.

Techniques: Staining, Immunofluorescence, Standard Deviation